Session I :
January 16, 2025
Going Back to Basics
Thank you Sponsors!
Bruno Humbel
Juntendo University in Tokyo
&
Max-Planck Institute of Polymer Science in Mainz
Biological Sample Preparation - Chemical Fixation, Cryo-Fixation, Freeze-Substitution
Bruno received a PhD in Cell Biology from the Federal Institute of Technology (ETH) in Zurich, where he worked in Dr. Martin Mueller's lab within the department led by Prof. Hans Moor, both pioneers in cryo-electron microscopy. Following this, Bruno joined the Max Planck Institute of Biochemistry in Munich under Prof. Guenter Gerisch. Four years later, Bruno was invited by Prof. Arie Verkleij to Utrecht University in the Netherlands, where he served as an Assistant and later Associate Professor.
After Prof. Verkleij's retirement, Bruno moved to the University of Lausanne, where he revitalized the electron microscopy lab previously led by Prof. Jacques Dubochet. Bruno then achieved a long-held dream by to go back to Japan by joining the Okinawa Institute of Science and Technology (OIST) in Japan as the head of the Imaging Lab. Bruno's primary research interests include biological sample preparation using cryo-fixation, correlative microscopy, and volume electron microscopy.
In April 2024, Bruno retired from OIST but continues to contribute to the field as a visiting professor at Juntendo University in Tokyo and the Max Planck Institute for Polymer Science in Mainz. He also teaches in his areas of expertise in Japan, China, Denmark, Germany, and Brazil.
Jesse Aaron
Tools for processing, visualization, and analysis of large 3D EM data
Jesse has nearly 20 years’ experience in microscope design, development, and application across a wide range of biological models. He received his Ph.D. in Biomedical Engineering from the University of Texas at Austin under the guidance of Konstantin Sokolov and Rebecca Richards-Kortum and performed postdoctoral work at Sandia National Laboratories in Albuquerque, NM, where he implemented several high-resolution optical imaging systems, including TIRF, STORM, hyperspectral, and STED microscopies, with biological applications ranging widely from cell surface receptor tracking and innate immune response, to algal biofuels research. Jesse joined the Advanced Imaging Center at Janelia in 2014, He is now the manager of the Integrated Imaging Technologies. Jesse has also been leading the AIC's efforts to disseminate Janelia's enhanced 3D FIB-SEM imaging technologies to researchers throughout the world.
Discussion Forum: EM Lab Safety & Chemical waste management