Session VI : November 16, 2023
Tackling Difficult Samples
Program for Session VI
Dr. Philipp Bastians
Sales Manager, Life Science Electron & X-Ray Microscopy, North America ZEISS Research Microscopy Solutions
Acquiring Volumes of Interest in Challenging EM Samples
Phillipp started his career with ZEISS as a Product and Applications Sales Specialist in the Asia Pacific Region after he graduated from Max-Planck-Institute for Brain Research, Frankfurt am Main, Germany with a PhD on “Comparative Cortical Connectomics”. His work includes many years of 3D SEM, cryo EM and MultiSEM imaging technology as well as X-Ray imaging.
Preparation of Bone for Transmission Electron Microscopy
Lee Cohen-Gould is the Senior Director of the Microscopy & Image Analysis Core Facility at Weill Cornell Medicine in New York City. Originally hired in 1988 to run what was then the Electron Microscopy Core, the facility has expanded to include optical microscopy instruments including wide field for histology and fluorescence to confocal, 2P, light sheet, whole slide scanning, high throughput and long-term incubation/time lapse imaging.
Lee has worked with a wide variety of samples ranging from tissues, to cell cultures & organoids to isolated proteins and extracellular vesicles. Bone samples are among the more challenging tissues submitted to the Core for both histology and electron microscopy.
Ultramicrotomy for Biological specimen
Helmut has a strong engineering background. He cofounded Diatome in 1970 and designed the machines and apparatus for manufacturing diamond knives for ultramicrotomy. Throughout the years, Helmut developed diamond knives for ultrathin sections, cryo knives for biological and polymer sectioning, oscillating diamond knives, antistatic devices, double manipulators and other accessories for ultramicrotomy. From 1990-2010, Helmut collaborated with Prof. J. Dubochet (winner of Nobel prize in 2017) and made significant contributions to the development of knives, antistatic devices, and manipulators for cryo ultramicrotomy. Helmut has been traveling and teaching ultramicrotomy courses for both material and biological sciences worldwide since the late 1970s. In 2016, he was elected a Fellow of the Microscopy Society of America. Helmut also co-authored ~ 30 scientific publications on ultramicrotomy, both in material and biology sciences.
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