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Session II : March 16, 2023
FE-SEM & FIB-SEM

Download  Program for Session II

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Lei Gao

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Lei Gao, Ph.D. is a Research Scientist at the Tsinghua University of China. She received a medical doctorate degree in Pathogen Biology from the Jilin University of China in 2012. She was a postdoc in Ultrastructural Pathology at Harbin Medical University of China from 2012 to 2014 and worked as a Deputy Director in the Center of Electron Microscopy at Harbin Medical University for 7 years. As Senior EM Technologist, Research Scientist of Microscopy Core Facility at the Westlake University of China for 3 years. She has over 10 years of experience in cutting-edge, shared-use microscopy facilities for biomedical research and teaching experience in a higher-education environment. Her research expertise is in 3D-SEM imaging of biological systems, including cells, macromolecular assemblies, and filaments. Especially, electron microscopy of cells and tissues to study the fine structure of different types of biological objects (plant, animal, cultured cells, isolated organelles, bacteria, fungus, viruses).

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Kedar Narayan

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Empanada: a tool to expand the FIB-SEM segmentation bottleneck

Kedar Narayan works as a senior scientist and group leader at the Center for Molecular Microscopy (CMM) at Frederick National Laboratory and National Cancer Institute, USA. Kedar has a Ph.D. in immunology, with an emphasis on biophysics and imaging, and a background in chemistry, pathology and software engineering. His group has developed and applied FIB-SEM and other volume EM technologies to questions in cell biology. Specific areas of focus include correlative imaging, deep learning/AI, tool development, and standards for the volume EM field. As a leading member of the volumeEM community, Kedar is committed to the growth and democratization of the field.

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Mike Reichelt

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Mike earned a PhD in Virology from the University of Freiburg / Germany and was a postdoctoral research scholar at Stanford University School of Medicine performing research in the field of viral pathogenesis. He made extensive use of cellular electron microscopy methods to study viral replication and cellular responses to viral infections at the ultrastructural level. In 2012, Mike joined the department of Pathology at Genentech, bringing with him his expertise in cellular electron microscopy. He works now in the role as a senior principle scientific researcher (Scientist IV) and runs the daily operation of the electron microscopy lab, which is a subgroup of the Center for Advanced Light and Electron microscopy (CALM-EM) within the Pathology Department at Genentech. He manages the daily operations of the pathology EM lab, which is a full service laboratory that supports scientists in the Genentech Research and Early Development organization (gRED). The EM group collaborates with scientist from nearly all departments in gRED, including research pathology, safety assessment, neuroscience, microbiology, immunology and experimental oncology.  A  wide range of different type of biological samples, including macromolecular protein assemblies, human pathogenic viruses and bacteria, cultured cells and various normal, diseased or experimental tissues of both animal or human origin are processed and investigated routinely.

Flash Talk

Automatic high throughput staining platform for EM grids with transferrable grid holder

Jingnan Liang

Senior Technologist, Core Facility at Institute of Microbiology 

Chinese Academy of Sciences

Beijing, China

Tech Talk

The Integration of X-Ray and Electron Microscopy: A Targeted Approach toward volume EM

Joe Mowery

Life Science EM/XRM Sales Specialist

Zeiss Research Microscopy Solutions

Carl Zeiss Microscopy, LLC

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